dextran hydrodynamic radiusdextran hydrodynamic radius

The human skins were from the back or thigh of a 24-year-old woman. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Biological skin substitutes for wound cover and closure. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. Bookshelf As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Journal of Controlled Release, 119, 5-24. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. Hydrodynamic Properties of Tragacanthin. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. J Cosmet Laser Ther. Determination of intrinsic viscosities of macromolecules and nanoparticles. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). official website and that any information you provide is encrypted Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Cookies policy. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. (1990). In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). The term f/f0 is sometimes denoted as P, Perrin constant. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. Pharm Res. Molecular weight (M v) and hydrodynamic properties can be calculated from there. Ahn, G.Y., Eo, HS., Kim, D. et al. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Food Funct. The micropores of the human cadaver skin were regenerated according to the time. Thus, it is a common practice to express the frictional coefficient of rigid structures as. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Polymer molecular weight distribution. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Probiotics Antimicrob Proteins. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Trop J Pharm Res. (1998). MeSH The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Crini, G. (2005). WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. American Journal of Food Science and Technology 2013, 1(3), 60-66. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. The above data are not useful because it can not be compared with those obtained in this work. ; Ghosh, T.K. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Ed., 9(2), 85-90. Learn More. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) where tr is the column retention time in minutes (2 of 0.9792). Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). Thermo Fisher Scientific. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. The hydrodynamic 57 radius of the dextranase is 7 Increased epidermal laser fluence through simultaneous ultrasonic microporation. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! (2013). Masuelli, Martin Alberto. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. ; Tsuchiya, H.M.; Rist, C.E. HSE and DK helped to porate the skin and to measure TEWL. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Polymolecularity and polydispersity in Molecular weight determinations. De Belder, A.N. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). Kumar SV, Tarun P, Kumar TA. Doke SK, Dhawale SC. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. government site. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). 2012;3(1):208. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. 1984;17(6):11703. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Structure Properties of Dextran. Appl Biochem Biotechnol. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. Degrees of long-chain branching in dextrans. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Drug Deliv. The enhanced permeability through micropores was confirmed by Franz diffusion cell. Figure4 shows the histological analysis of micropores in human cadaver skin. Nordmeier, Eckliard (1993). For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. 151-166. (2006). International Journal of Pharmaceutics, 277, 99-104. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Citation-(RIS WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. The https:// ensures that you are connecting to the Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Radiofrequency with microneedling. Google Scholar. 2013, Article ID 360239, pp. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. J. Braz. Federal government websites often end in .gov or .mil. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). The micropore formed at the SC through to the epidermis, enabling drug delivery. B 113, 8020-8025. Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. Colloid and Polymer Science, 286, 1223-1231. Kasaai, Mohammad R. (2012). what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the WebTry out PMC Labs and tell us what you think. 2012;9(2):14754. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. eCollection 2018 Feb. Molecules. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. NCI CPTC Antibody Characterization Program. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta ; Ross-Murphy, S.B. Format), Citation-(EndNote Expert Opin Drug Deliv. Exp Cell Res. Microneedles and their applications. Fixable: Fixable via Lysine. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. WebTry out PMC Labs and tell us what you think. Carbohydrate Polymers 99, 199-207. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. 2005;22(4):5505. Unauthorized use of these marks is strictly prohibited. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. Careers. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. (1999). Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Granath, K. A. Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. (2003). Porcine wound models for skin substitution and burn treatment. ; Dutt, N.V.K. Epub 2012 Oct 18. and transmitted securely. 4K, b M.W. FITC-dextran solution (600L) was added to the donor chamber. Pharm Res. ; Bataille, B. 2013;43(4):287303. Ioan, C.E. Biomaterials, 22, 1891-1898. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Sachdeva V, Banga AK. ; Prasad, D.H.L. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Guner, A.; Kibarer, G. (2001). Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. One day before the experiment, the skin was stabilized in the skin culture medium. (1954). A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. pp13-21. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Intrinsic viscosity of bovine serum albumin conformers. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. (2008). Terms and Conditions, The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Indo AM J Pharm. Format), Citation-(BibTeX DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. (1937). For data requests, please contact the authors. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. Webof overall macromolecular geometry and hydrodynamic draining. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Weissella: An Emerging Bacterium with Promising Health Benefits. Formation of dextran hydrogels by crystallization. H&E stained images were analyzed using Image J software. Chem. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. J. Braz. (2000). Chem. Saudi Pharm J. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Drug Discov Today Technol. Sung-Wook Choi. This situation is mainly the result of using dextans, solvent, additives, etc. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. 4h after microporation, the sizes of the micropore decreased slightly. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. Schematic illustration of transdermal delivery after the application of the microporation device. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. dextran hydrodynamic radius. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. They concluded that a power law behavior seems to be approached only in the small molecular weight region. Biomacromolecules 8, 2464-2475. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. 2004;25(9):155967. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. Format). Formul. Google Scholar. Cite this article. Carbohydrate Polymers 88 373-381. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. (2003). Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Pharm Res. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. (2003). Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. 2019;9(3-S):9726. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. J Control Release. TEWL measurements were performed before and 30min after microporation. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The hydrodynamic radius of 2-NBDG glucose was too small to measure. 2. SWC designed and coordinated the research. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Table 2 compares the properties of nanoparticles coated with one or other polymer. (1999). 2008;28(5):64557. The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. PMC Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Ind. 2003;89(2):31120. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc.
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